Isolation and Identification of Predominate Urinary Tract Infection Bacteria and Assessed Their Chemotactic Behavior in the Presence of Some Medicinal Herbal Plant Extracts

Zahra moradi, Majid Baserisalehi and Nima bahador

Abstract:

The Purpose of the study was the isolation and identification of Urinary tract infection (UTI) bacteria and the evaluation of their susceptibility against antibiotics. In addition, susceptibility and chemotactic behavior of isolated strains were evaluated against medicinal herbal plant extracts.

To perform the study, 200 samples were collected from the patients suffering from UTI. Then, the bacterial agents were isolated and their susceptibility were assessed against gentamicin, ciprofloxacin, nitrofurantoin, trimethoprim-sulfamethoxazole, nalidixic acid, cephalexin, cefixime and ceftriaxone by disk diffusion method. In addition, Thymus vulgaris, Achilleamillefolium, Teucriumpolium, Origanumvulgare, Salvia officinalis, Urticadioica, Matricariachamomilla, Foeniculumvulgare, Allium ascalonicum and Cinnamomumverum were extracted using boiling water and ethyl alcohol (70%) and their antimicrobial effects were tested against isolated strains by Well Agar Diffusion method. Finally, chemotaxis behavior of some isolates evaluated against sub Arbitrary concentration of effective medicinal herbal plants. The results obtained indicated that Gram negative and Gram positive bacteria were isolated with a frequency of 80% and 20% respectively. Escherichia coli and Staphylococcus saprophyticus were predominant agents. Moreover, ciprofloxacin, nitrofurantoin, and gentamicin exhibited more effectiveness. Out of all drug plant extracts, Allium ascalonicum and Cinnamomumverum had exhibiting relatively more antimicrobial effect against urinary tract infection agents. Our finding, concerning to chemotactic behavior of motile bacterial strains indicated that both herbal medicinal plants Allium ascalonicum and Cinnamomumverum eliminated chemotactic behavior of Escherichia coli, proteusmirabilis and Enterobactersakazaki. Hence, due to increasing level of antibiotic resistant bacteria, which causing UTI, some medicinal herbal plants such as Allium ascalonicum and Cinnamomumverum could be considered as a new source of remedy for treatment of the patients suffering from the infection.

Keywords: Urinary tract infection, chemotactic behavior, medicinal herbal plant.

Pages: 149 – 156 | Full PDF Paper