In Vitro Mass Multiplication and Isolation of Natural Products from Callus Tissues of Salvia santolinifolia (Boiss)
Tour Jan, Beena Naqvi and Raiha Qadri
Abstract: Method for mass multiplication of callus of Salvia santolinifolia by nodal explants was investigated by optimizing the concentration and combinations of different plant growth regulators in MS medium. Nodal explants could be stimulated to form callus on MS medium supplemented with 1-Naphthalene acetic acid (NAA) (0.1, 0.5 and 1.0 mg/l). Large scale multiplication of callus was achieved on MS medium containing NAA (0.3 mg/l) plus N6 Benzylaminopurine (BAP) (1.5 mg/l) and NAA (0.3 mg/l) plus N6-(2-isopentyl)-adenine (2iP) (1.0 mg/l). The phytochemical investtigation of callus lead to the isolation of b-Sitosterol and Stigmasterol compounds.
Keywords: Callus, mass multiplication, salvia, Lamiaceae.
Pages: 320 – 328 | Full PDF Paper