1. CNI-1493 Administration Improves the Efficacy of Cytotoxic T Lymphocytes

    Salem Ramadan Sariti, Sabine Specht and Achim Hoerauf


    CNI-1493, is a synthetic guanylhydrazone with anti-inflammatory properties. The most important application of CNI-1493 is the treatment of Crohn’s disease. Adenovirus expressing ovalbumin (AdOVA) induces an increase, as a short peak, of IL-12 shortly after administration, and increased ovalbumin-specific cytotoxic T lymphocyte cells.

    Aim of study: To evaluate the safety and immunological effects of the CNI-1493 on the in vivo activity of cytotoxic T lymphocyte cells (CTLs), by using AdOVA immunized C57Bl/6 mice.

    Materials and methods: AdOVA were given i.v. at the same time point to two groups of naïve C57Bl/6 WT mice; Gp1: Received CNI-1493, AdOVA and target cells and Gp2: Received AdOVA and target cells. In addition to the above two groups, a control group (Gp3): Received target cells (Neither CNI-1493 nor AdOVA). Five hours, after the target cells administration, the spleens were taken out of the experimental groups and homogenised in 1xPBS. The ratio of lysed target cells was determined by flow cytometry

    Results: The OVA specific lysis of target cells in immunized C57Bl/6 mice of the experimental groups were 47.2% in G1 and 81.3%  in G2.

    Conclusion: CNI-1493 is compound which is safe and undamaging to the immunological function of the cytotoxic T lymphocyte cells.

    Keywords: CNI-1493, ovalbumin, specific cytotoxic T lymphocyte cells, and immunized C57Bl/6 mice.

    Pages: 329 – 337 | Full PDF Paper
  2. Extreme High Count Rate Performance with a Silicon Drift Detector and ASIC Electronics

    S. Barkan, V.D. Saveliev, Y. Wang, L. Feng, E.V. Damron, Y. Tomimatsu

    Abstract: Extremely High Count-Rate Performance of Silicon Drift Detector (SDD), the Vortex®, has been achieved by integrating it with new front-end ASIC electronics and using a new pulse processing technology. The use of ASIC electronics produced a very low input capacitance that also significantly improved resolution at short peakingtimes.

    Pages: 338 – 344 | Full PDF Paper
  3. Evaluation of a Nipple Sealer Based on Brad Infusion

    Sofía Milagros Leyton, Dr. Juan B. Beltramino

    Abstract: This research work was performed to replace the antimicrobials that are usually used in the trading nipples’ sealants, changing them by a local herbalist resource, the Brad (Erodium cicutarium), as an infusion in the active ingredient and this way detecting new antimicrobial fonts. To evaluate its efficiency, in-vitro analysis was made at school laboratory as well as in the Pharmacognosy Class of the Faculty of Pharmacy and Biochemistry at the Universidad de Buenos Aires, Argentina. Since April 2013 until September 2013, fieldwork was made over bovines at Escuela Agropecuaria Provincial N°1 in Gobernador Gregores, Santa Cruz, Argentina. At in-vitro trials, the results obtained using sealer with brad as an infusion basis, showed a good antiseptic behavior as it reduced in more than 5 logarithms the units maker of colonies, coming from the nipple’s skin. At fieldwork, after 21 days of application, it reduced the bacterial load of the nipple’s skin in a statistically significant way (p < 0.001). A TLC analysis detected that in the brad infusion existed some kinds of flavonoids such as: rutin and other glycosides from quercetine. An HCPL analysis confirmed the presence of phenolic compounds such as quercitine, rutin, chlorogenic acid, isoquercitrine and quercitrine. The cost of the elaborated sealer was up to 30% lower than the trading ones.

    Keywords: Nipple sealer, Brad, Antiseptics.

    Pages: 345 – 355 | Full PDF Paper