Identification of Mannose-Binding Protein from Milkfish (Chanos chanos F.) Serum
Anacleto M. Argayosa, Joshua Rovie L. Daclan, Miguel S. Acuin, Ma. Lourdes Nilles-Bije
Abstract: Lectins are integral part of the innate immunity of vertebrates performing roles in cell recognition and the neutralization of pathogens through protein-carbohydrate interactions. Mannose-binding protein, a type of lectin, isolated from the sera of milkfish by affinity chromatography was subjected to molecular weight analysis using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). The protein bands showed molecular weights of 55 kDa, 30kDa and 12 kDa under reducing condition. Non-reducing and native PAGE conditions suggest multimeric structure of the 30 kDa MBP. The 55kDa and 12 kDa MBPs are possibly other mannose-binding proteins or co-eluting proteins that require further analyses to understand their characteristics and properties. A 50 kDa MBP showed strong agglutinating activity against Candida albicans suggesting the presence of mannan moieties on the surface of the yeast cell.
Keywords: Fish innate immunity, Mannose-binding protein, Affinity chromatography, SDS-PAGE, Candida albicans.
Pages: 277 – 288 | Full PDF Paper