• Intensification of phenolic compounds extraction from Nitraria retusa leaves by Ultrasound-Assisted System Using Box–Behnken Design and Evaluation of Biological Activities

    Feten Zar Kalai1., Khaoula Mkadmini1, Majdi Hammami1, Hiroko Isoda2.3 and Riadh Ksouri1

    1. Laboratory of Aromatic and Medicinal Plants, Center of Biotechnology, Technopark of Borj Cedria, BP 901, 2050 Hammam-Lif, Tunisia.
    2. Alliance for Research On the Mediterranean and North Africa (ARENA), University of Tsukuba, Tsukuba, Japan.
    3. Faculty of Life and Environmental Sciences, University of Tsukuba, 1 1 1 Tennodai, Tsukuba, Ibaraki 305 8572, Japan.

    Abstract: The aim of this work is to optimize the antioxidant extraction conditions from leaves of N. retusa with an ultrasound-assisted system using response surface methodology. The Box-Behnken design was employed for optimization of extraction parameters in terms of antioxidant activities using 2,2-diphenyl-1-picrylhydrazyl radical scavenging activity and total phenolic content. The optimum operating conditions for extraction were as following: ethanol concentration, 75.58 %v/v; extraction time, 5.99 min; extraction temperature, 42.15°C. Under these conditions, the obtained extract exhibited a high content of phenolic compounds (24 mg EAG/g DR) with potent antioxidant activity (DPPH: CI50= 7 µg/ml; FRAP: EC50=55 µg/ml). The optimum extract exhibited high anti-inflammatory activity with no cytotoxicity which could be attributed to the synergistic action of the identified glycosylated flavonoids (Luteolin 7-O-glucoside, Rutin, Kaempferol 3-O-rutinoside, Isorhamnetin 3-O-rutinoside).

    Keywords: Nitraria retusa, ultrasound-assisted system, Box-Behnken, antioxidant activities, biological activities.

    Pages: 19 – 34 | Full PDF Paper