Evaluation of a Nipple Sealer Based on Brad Infusion
Sofía Milagros Leyton, Dr. Juan B. Beltramino
Abstract: This research work was performed to replace the antimicrobials that are usually used in the trading nipples’ sealants, changing them by a local herbalist resource, the Brad (Erodium cicutarium), as an infusion in the active ingredient and this way detecting new antimicrobial fonts. To evaluate its efficiency, in-vitro analysis was made at school laboratory as well as in the Pharmacognosy Class of the Faculty of Pharmacy and Biochemistry at the Universidad de Buenos Aires, Argentina. Since April 2013 until September 2013, fieldwork was made over bovines at Escuela Agropecuaria Provincial N°1 in Gobernador Gregores, Santa Cruz, Argentina. At in-vitro trials, the results obtained using sealer with brad as an infusion basis, showed a good antiseptic behavior as it reduced in more than 5 logarithms the units maker of colonies, coming from the nipple’s skin. At fieldwork, after 21 days of application, it reduced the bacterial load of the nipple’s skin in a statistically significant way (p < 0.001). A TLC analysis detected that in the brad infusion existed some kinds of flavonoids such as: rutin and other glycosides from quercetine. An HCPL analysis confirmed the presence of phenolic compounds such as quercitine, rutin, chlorogenic acid, isoquercitrine and quercitrine. The cost of the elaborated sealer was up to 30% lower than the trading ones.
Keywords: Nipple sealer, Brad, Antiseptics.
Pages: 345 – 355 | Full PDF Paper