• Problems in Breastmilk Cell Isolation

    Sri Lilijanti Widjaja1, Harsono Salimo2, Suradi3, and Indah Yulianto4

    1. Student at Doctoral Program of Medical Sciences, Pediatric Department Dr. Moewardi Hospital, Faculty of Medicine Universitas Negeri Sebelas Maret, 57126, Surakarta, Indonesia.
    2. Pediatric Department Dr. Moewardi Hospital, Faculty of Medicine Universitas Negeri Sebelas Maret, 57126, Surakarta, Indonesia.
    3. Pulmonology Department Dr. Moewardi Hospital, Faculty of Medicine Universitas Negeri Sebelas Maret, 57126, Surakarta, Indonesia.
    4.Dermatology and Venereology Department Dr. Moewardi Hospital, Faculty of Medicine Universitas Negeri Sebelas Maret, 57126, Surakarta, Indonesia.

    Abstract: Background: Breastmilk contains a plethora of bioactive factors, including biochemical and cellular components. Maternal cells in breastmilk comprise a heterogeneous population of epithelial cells, leukocytes, progenitor and stem cells that dynamically respond to maternal and infant needs. Previous studies have confirmed the presence of many types of cells in the human breastmilk cells. Breastmilk cells is susceptible and only appear in small number in the breastmilk. Although cellular analyses have been previously conducted in breastmilk, optimization of the methodology to isolate and process cells from breastmilk is lacking. In this study, we aim to elucidate the problems in breastmilk cell isolation using two culture media. Methods: We isolate human breastmilk cell using two different media, DMEM with fetal bovine serum (FBS) and MammocultTM. Human breastmilk was prepared and processed using protocol from previous study and then cultured into two different media. Results: We faced some problems in breastmilk cell isolation process with two media. Breastmilk sample preparation, reagents use, and time of isolation process is major factor which determine cell viability in breastmilk cell culture. Conclusion: Error in breastmilk sample preparation, time consuming isolation process, and breastmilk sample volume may affect the breastmilk cell viability.

    Keywords: breastmilk cell, isolation, cell culture.

    Pages: 163 – 171 | Full PDF Paper