Biological Activities of Endophytic Xylaria sp. Isolated from Tropical forest in Chaiyapoom Province, Thailand

Krittapong Orachaipunlap, Nuttika Suwannasai, Anthony J.S. Whalley, Cherdchai Phosri4, Prakitsin Sihanonth

Abstract: The ultimate goal in this study is to screen biological activities from endophytic Xylaria sp. isolated from leaf of 4 Dipterocarp forest tree species such as Cinnamomum iners, Shorea siamensis, Fermandoa adenophylla, Quercus semiserrata. All endophytic fungi isolates were obtained and identified based on morphological characteristics. Sixty-four from 125 endophyte isolates were belong to Xylaria genera. Moreover, the typical endophytic fungi genera such as Phomopsis spp., Pestalotiopsis sp., Colletotrichum sp., Phyllosticta sp., Daldinia sp., Aspergillus sp., Mycelia sterilia spp., were commonly found in tropical forest. Xylaria isolate sp.1 was the only one isolate that showed excellent broad spectrum antimicrobial against Bacillus subtilis, Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli, and Candida albicans. Moreover, Xylaria isolate sp.9 showed strong inhibitory activity against all test bacteria. Moreover, Xylaria sp.1 showed the best efficiency of cytotoxicity against Jurkat cell line with IC₅₀ value of 2.63 μg/mL and Xylaria sp.2 showed best efficiency of cytotoxicity against HEK293 cell line with IC₅₀ value of 2.94 μg/mL..

Keywords: Endophytic fungi, Xylaria sp., Antimicrobial activity, Anticancer activity, Bioactive compound.

Pages: 200 – 208 | Full PDF Paper